Waiting
로그인 처리 중...

Trial ends in Request Full Access Tell Your Colleague About Jove
JoVE Journal
Biology

JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.

Lentiviral-mediated Knockdown During Ex Vivo Erythropoiesis of Human Hematopoietic Stem Cells
 
Click here for the English version

Lentiviral-mediated Knockdown During Ex Vivo Erythropoiesis of Human Hematopoietic Stem Cells

Article DOI: 10.3791/2813-v 14:23 min July 16th, 2011
July 16th, 2011

챕터

요약

Please note that all translations are automatically generated.

Click here for the English version.

An ex vivo protocol to generate mature human red blood cells from hematopoietic stem/progenitors is described. Additionally we describe an efficient lentiviral-delivery method to knockdown the transcription factor TAL1 in primary erythroid cells. The efficiency of lentivirus mediated gene delivery is demonstrated using GFP expressing viruses.

Tags

Lentiviral-mediated Knockdown Ex Vivo Erythropoiesis Human Hematopoietic Stem Cells Cell Differentiation Gene Expression Transcription Factors Lineage-specific Genes Cell Lines Erythroid Cells Stages Of Differentiation Primary HSCs Erythroid Differentiation Cord Blood Bone Marrow Adult Peripheral Blood
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter