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Journal / Bioengineering / Optical Mapping of Action Potentials and Calcium…
JoVE Journal
Bioengineering

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Optical Mapping of Action Potentials and Calcium Transients in the Mouse Heart

Article DOI: 10.3791/3275-v • 08:13 min • September 13th, 2011
September 13th, 2011 •
1, 1, 1, 1
1Department of Biomedical Engineering, Washington University in St. Louis

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This paper details the dissection procedure, instrumental setup, and experimental conditions during optical mapping of transmembrane potential (Vm) and intracellular calcium transient (CaT) in intact isolated Langendorff perfused mouse hearts.

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Optical Mapping Action Potentials Calcium Transients Mouse Heart Cardiovascular Studies Fluorescence Imaging Genetic Engineering Physiological Phenotyping Transmembrane Potential (Vm) Excitation-contraction Coupling Langendorff Perfused Mouse Hearts Heart Failure Arrhythmias Metabolic Disease Heart Diseases Activation Visualization Conduction Velocity Action Potential Duration Instrumentation Setup Experimental Conditions Spatio-temporal Resolution CMOS Imaging Technology
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