JoVE Journal
Biology
Biology
이 콘텐츠 오픈 액세스로 구독없이 시청하실 수 있습니다.
챕터
요약
This five-day protocol outlines all steps, equipment, and supplemental software necessary for creating and running an efficient endogenous Escherichia coli based TX-TL cell-free expression system from scratch. With reagents, the protocol takes 8 hours or less to setup a reaction, collect, and process data.