Mass Spectrometric Approaches to Study Protein Structure and Interactions in Lyophilized Powders

Balakrishnan S. Moorthy; Lavanya K. Iyer; Elizabeth M. Topp

doi:10.3791/52503

Journal

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화학

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질량 분석은 동결 건조 분말에서 단백질의 구조와 상호 작용을 연구하는 접근

JoVE 신문
화학

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JoVE 신문 화학

Mass Spectrometric Approaches to Study Protein Structure and Interactions in Lyophilized Powders

질량 분석은 동결 건조 분말에서 단백질의 구조와 상호 작용을 연구하는 접근

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15,576 Views

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11:14 min

•

April 14, 2015

DOI:

10.3791/52503-v

DOI:

10.3791/52503-v

•

11:14 min

April 14, 2015

•

5 Views

Balakrishnan S. Moorthy*¹, Lavanya K. Iyer*¹, Elizabeth M. Topp¹

¹Department of Industrial and Physical Pharmacy,Purdue University

Summary

Here, we present detailed protocols for solid-state amide hydrogen/deuterium exchange mass spectrometry (ssHDX-MS) and solid-state photolytic labeling mass spectrometry (ssPL-MS) for proteins in solid powders. The methods provide high-resolution information on protein conformation and interactions in the amorphous solid-state, which may be useful in formulation design.