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An In Vitro Dormancy Model of Estrogen-sensitive Breast Cancer in the Bone Marrow
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An In Vitro Dormancy Model of Estrogen-sensitive Breast Cancer in the Bone Marrow: A Tool for Molecular Mechanism Studies and Hypothesis Generation

Article DOI: 10.3791/52672-v • 08:48 min • June 30th, 2015
June 30th, 2015 •
1, 1, 1, 1
1Department of Medicine and New Jersey Medical School Cancer Center, Rutgers New Jersey Medical School

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요약
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We developed an in vitro model of dormancy in the bone marrow for estrogen-sensitive breast cancer cells. The goal of this protocol is to demonstrate use of the model for the study of the molecular and cellular biology of dormancy and for generation of hypotheses for subsequent testing in vivo.

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Breast Cancer Bone Marrow Dormancy Model Estrogen-sensitive Molecular Mechanism Studies Hypothesis Generation In Vitro Clonogenic Model Microenvironment Hematopoietic Cells Estrogen Sensitive Breast Cancer Cells Incubation Clonogenic Density Fibronectin FGF-2 Dormant Clones Flat Appearance Cytoplasm To Nucleus Ratio
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