Establishment of Genome-edited Human Pluripotent Stem Cell Lines: From Targeting to Isolation

John D. Blair; Helen S. Bateup; Dirk F. Hockemeyer

doi:10.3791/53583

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게놈 편집 인간 다 능성 줄기 세포의 설립 : 분리에 타겟팅에서

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Establishment of Genome-edited Human Pluripotent Stem Cell Lines: From Targeting to Isolation

게놈 편집 인간 다 능성 줄기 세포의 설립 : 분리에 타겟팅에서

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13,439 Views

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09:51 min

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February 02, 2016

DOI:

10.3791/53583-v

DOI:

10.3791/53583-v

•

09:51 min

February 02, 2016

•

3 Views

John D. Blair¹, Helen S. Bateup¹, Dirk F. Hockemeyer¹

¹Department of Molecular and Cell Biology,University of California, Berkeley

내레이션 대본

Summary

Genome editing of human pluripotent stem cells (hPSCs) can be done quickly and efficiently. Presented here is a robust experimental procedure to genetically engineer hPSCs as exemplified by editing the AAVS1 safe harbor locus to express EGFP and introduce antibiotic resistance.