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Journal / Neuroscience / 设计，包装，并通过立体定向注射高效价CRISPR复古和慢交付…
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设计，包装，并通过立体定向注射高效价CRISPR复古和慢交付
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设计，包装，并通过立体定向注射高效价CRISPR复古和慢交付

Article DOI: 10.3791/53783-v • 11:28 min • May 23rd, 2016
May 23rd, 2016 •
1, 1, 1, 1, 1
1Department of Physiology and Neurobiology, Geisel School of Medicine at Dartmouth College

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该CRISPR / Cas9系统提供作出有针对性的基因组编辑获得和负担得起科学界的潜力。该协议旨在演示如何创建病毒将基因敲除使用CRISPR / Cas9系统感兴趣的基因，然后立体定位将它们注入成年小鼠大脑。

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神经科学，第111，慢病毒，逆转录病毒，病毒包装，滴度高，
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