탠덤 선호도 태그 방법을 사용하여 구조 연구에 대한 기본 공단의 정화
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10:36 min
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July 27, 2016
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Summary
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The Tandem Affinity Purification (TAP) method has been used extensively to isolate native complexes from cellular extract, primarily eukaryotic, for proteomics. Here, we present a TAP method protocol optimized for purification of native complexes for structural studies.
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Cite this Article
van der Feltz, C., Pomeranz Krummel, D. Purification of Native Complexes for Structural Study Using a Tandem Affinity Tag Method. J. Vis. Exp. (113), e54389, doi:10.3791/54389 (2016).
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