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Harvest of Endothelial Cells from the Balloon Tips of Swan-Ganz Catheters after Right Heart Catheterization
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Harvest of Endothelial Cells from the Balloon Tips of Swan-Ganz Catheters after Right Heart Catheterization

Article DOI: 10.3791/58353-v • 07:45 min • January 23rd, 2019
January 23rd, 2019 •
1, 1, 1, 1, 1
1Cardiovascular Institute, Allegheny Health Network

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요약
Automatic Translation

This protocol describes the removal and purification of pulmonary artery endothelial cells (PAECs) that adhere to the balloon tips of Swan-Ganz catheters while they are wedged in the pulmonary artery during right heart catheterization and remain adherent to the deflated balloon following removal from the patient's body.

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Harvest Endothelial Cells Balloon Tips Swan-Ganz Catheters Right Heart Catheterization Molecular Path Intimal Proliferation Pulmonary Hypertension Cellular Biopsy Risk To The Patient Clinical Coordinator Laboratory Manager Centrifuge Tube Sterile Saline Forceps Endothelial Cell-growth Medium Wet Ice Overnight Shipping
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