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Journal / Biochemistry / Strategisk screening och karakterisering av…
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Biochemistry

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JoVE Journal Biochemistry
Strategisk screening och karakterisering av Visual GPCR-mini-G Protein Signaling Complex för framgångsrik kristallisering
Automated Protocols for Macromolecular Crystallization at the MRC Laboratory of Molecular Biology
Journal (Biochemistry)
Automated Protocols for Macromolecular Crystallization at the MRC Laboratory of Molecular Biology
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Journal (Biochemistry)
Illuminating New Avenues for Adipose Tissue Metabolism and Disease Prevention (Video) | JoVE
 
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Strategisk screening och karakterisering av Visual GPCR-mini-G Protein Signaling Complex för framgångsrik kristallisering

Article DOI: 10.3791/60747-v • 09:19 min • March 16th, 2020
March 16th, 2020 •
1,2, 1, 3, 4, 4, 4, 1
1Laboratory of Biomolecular Research, Paul Scherrer Institute, 2Department of Biology, ETH Zürich, 3Center for Radiopharmaceutical Sciences, Paul Scherrer Institute, 4Laboratory of Molecular Biology, Medical Research Council

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Denna rapport beskriver screening av olika rengöringsmedel för att förbereda den visuella GPCR, rhodopsin, och dess komplex med mini-Go. Biokemiska metoder som kännetecknar komplexets kvalitet i olika skeden under rening visas. Detta protokoll kan generaliseras till andra membran proteinkomplex för deras framtida strukturella studier.

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Biokemi Utgåva 157 tvättmedel rening rhodopsin-mini-Go komplex stabilitet glykosylering retinal SEC UV-VIS spektroskopi SDS-SIDA LC-MS
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