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Journal / Biochemistry / Strategisk screening og karakterisering af Visual …
JoVE Journal
Biochemistry

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Strategisk screening og karakterisering af Visual GPCR-mini-G Protein Signaling Complex for vellykket krystallisering
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Strategisk screening og karakterisering af Visual GPCR-mini-G Protein Signaling Complex for vellykket krystallisering

Article DOI: 10.3791/60747-v • 09:19 min • March 16th, 2020
March 16th, 2020 •
1,2, 1, 3, 4, 4, 4, 1
1Laboratory of Biomolecular Research, Paul Scherrer Institute, 2Department of Biology, ETH Zürich, 3Center for Radiopharmaceutical Sciences, Paul Scherrer Institute, 4Laboratory of Molecular Biology, Medical Research Council

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Denne rapport beskriver screening af forskellige rengøringsmidler til forberedelse af den visuelle GPCR, rhodopsin, og dens kompleks med mini-Go. Biokemiske metoder, der karakteriserer kompleksets kvalitet på forskellige stadier under rensningen, påvises. Denne protokol kan generaliseres til andre membran protein komplekser for deres fremtidige strukturelle undersøgelser.

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Biokemi vaskemiddel rensning rhodopsin-mini-Go kompleks stabilitet glycosylation retinal SEC UV-VIS spektroskopi SDS-PAGE LC-MS
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