Name: 약용 식물에서 트로판 알칼로이드의 정량을 위한 효율적이고 빠른 HPLC-QQQ-MS 방법 (동영상) | JoVE Journal
Uploaded: 2026-06-12T13:00:00.000+00:00
Duration: 9 min 10 s
Description: 학습하세요 약용 식물 Anisodus tanguticus에서 트로판 알칼로이드를 HPLC-QQQ-MS로 정량하는 고속 분석법을. 이 방법은 기존 HPLC의 민감도 한계를 극복하고 20분 이내에 정확한 정량이 가능합니다.

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5 minutes

<ol><li>Ji, P., Yang, X., Zhao, X. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Application+of+metabolomics+in+quality+control+of+traditional+Chinese+medicines%3A+a+review%5BTitle%5D)+AND+%22Front+Plant+Sci%22%5BJournal%5D)">Application of metabolomics in quality control of traditional Chinese medicines: a review.</a> Front Plant Sci. 15, 1463666(2024).</li>
<li>Li, S. -S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Phytochemical+variation+among+the+traditional+Chinese+medicine+mu+dan+pi+from+Paeonia+suffruticosa+%28tree+peony%29%5BTitle%5D)+AND+%22Phytochemistry%22%5BJournal%5D)">Phytochemical variation among the traditional Chinese medicine mu dan pi from Paeonia suffruticosa (tree peony).</a> Phytochemistry. 146, 16-24 (2018).</li>
<li>Wang, N., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Pinelliae+rhizoma+alleviated+acute+lung+injury+induced+by+lipopolysaccharide+via+suppressing+endoplasmic+reticulum+stress-mediated+NLRP3+inflammasome%5BTitle%5D)+AND+%22Front+Pharmacol%22%5BJournal%5D)">Pinelliae rhizoma alleviated acute lung injury induced by lipopolysaccharide via suppressing endoplasmic reticulum stress-mediated NLRP3 inflammasome.</a> Front Pharmacol. 13, 883865(2022).</li>
<li>Pughaih, T., et al. <a target="_blank" href="http://scholar.google.com/scholar?hl=en&safe=off&q=author%3aPughaih%2C+T+%22Tropane+Alkaloids%3A+Sources%2C+Chemistry%2C+Pharmacology+and+Biotechnology%22">Tropane Alkaloids: Sources, Chemistry, Pharmacology and Biotechnology</a>. , Nature, Springer. Singapore. (2025).</li>
<li>Chen, C., Wang, B., Xiao, Y., Xiong, F., Zhou, G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Geographical+origin+discrimination+of+Anisodus+tanguticus+%28Maxim.%29%3A+chemometrics+methods+applied+to+ICP-OES+analysis%5BTitle%5D)+AND+%22Arab+J+Chem%22%5BJournal%5D)">Geographical origin discrimination of Anisodus tanguticus (Maxim.): chemometrics methods applied to ICP-OES analysis.</a> Arab J Chem. 17 (5), 105730(2024).</li>
<li>Cardillo, R., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Scopolamine%2C+anisodamine+and+hyoscyamine+production+by+Brugmansia+candida+hairy+root+cultures+in+bioreactors%5BTitle%5D)+AND+%22Process+Biochem%22%5BJournal%5D)">Scopolamine, anisodamine and hyoscyamine production by Brugmansia candida hairy root cultures in bioreactors.</a> Process Biochem. 45 (9), 1577-1581 (2010).</li>
<li>Yuan, B., Zheng, C., Teng, H., You, T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Simultaneous+determination+of+atropine%2C+anisodamine%2C+and+scopolamine+in+plant+extract+by+nonaqueous+capillary+electrophoresis+coupled+with+electrochemiluminescence+and+electrochemistry+dual+detection%5BTitle%5D)+AND+%22J+Chromatogr+A%22%5BJournal%5D)">Simultaneous determination of atropine, anisodamine, and scopolamine in plant extract by nonaqueous capillary electrophoresis coupled with electrochemiluminescence and electrochemistry dual detection.</a> J Chromatogr A. 1217 (1), 171-174 (2010).</li>
<li>Zhang, P., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Simultaneous+determination+of+atropine%2C+scopolamine%2C+and+anisodamine+from+Hyoscyamus+niger+L.+in+rat+plasma+by+high-performance+liquid+chromatography+with+tandem+mass+spectrometry+and+its+application+to+a+pharmacokinetics+study%3A+liquid+chromatography%5BTitle%5D)+AND+%22J+Sep+Sci%22%5BJournal%5D)">Simultaneous determination of atropine, scopolamine, and anisodamine from Hyoscyamus niger L. in rat plasma by high-performance liquid chromatography with tandem mass spectrometry and its application to a pharmacokinetics study: liquid chromatography.</a> J Sep Sci. 37 (19), 2664-2674 (2014).</li>
<li>Chen, C., Wang, B., Li, J., Xiong, F., Zhou, G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Multivariate+statistical+analysis+of+metabolites+in+Anisodus+tanguticus+%28Maxim.%29+Pascher+to+determine+geographical+origins+and+network+pharmacology%5BTitle%5D)+AND+%22Front+Plant+Sci%22%5BJournal%5D)">Multivariate statistical analysis of metabolites in Anisodus tanguticus (Maxim.) Pascher to determine geographical origins and network pharmacology.</a> Front Plant Sci. 13, 927336(2022).</li>
<li>Chen, C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Multivariate+statistical+analysis+of+tropane+alkaloids+in+Anisodus+tanguticus+%28Maxim.%29+Pascher+from+different+regions+to+trace+geographical+origins%5BTitle%5D)+AND+%22Acta+Chromatogr%22%5BJournal%5D)">Multivariate statistical analysis of tropane alkaloids in Anisodus tanguticus (Maxim.) Pascher from different regions to trace geographical origins.</a> Acta Chromatogr. 34 (4), 422-429 (2022).</li>
<li>Sawabe, Y., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Rapid+determination+of+atropine+and+scopolamine+content+in+Scopolia+extract+powder+by+HPLC%5BTitle%5D)+AND+%22J+Nat+Med%22%5BJournal%5D)">Rapid determination of atropine and scopolamine content in Scopolia extract powder by HPLC.</a> J Nat Med. 65 (2), 395-399 (2011).</li>
<li>Baj, J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Application+of+HPLC-QQQ-MS%2FMS+and+new+RP-HPLC-DAD+system+utilizing+the+chaotropic+effect+for+determination+of+nicotine+and+its+major+metabolites+cotinine%2Cand+trans-3%E2%80%B2-hydroxycotinine+in+human+plasma+samples%5BTitle%5D)+AND+%22Molecules%22%5BJournal%5D)">Application of HPLC-QQQ-MS/MS and new RP-HPLC-DAD system utilizing the chaotropic effect for determination of nicotine and its major metabolites cotinine,and trans-3′-hydroxycotinine in human plasma samples.</a> Molecules. 27 (3), 682(2022).</li>
<li>Chen, D., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Qualitative+and+quantitative+analysis+of+the+major+constituents+in+Shexiang+tongxin+dropping+pill+by+HPLC-Q-TOF-MS%2FMS+and+UPLC-QqQ-MS%2FMS%5BTitle%5D)+AND+%22Molecules%22%5BJournal%5D)">Qualitative and quantitative analysis of the major constituents in Shexiang tongxin dropping pill by HPLC-Q-TOF-MS/MS and UPLC-QqQ-MS/MS.</a> Molecules. 20 (10), 18597-18619 (2015).</li>
<li>Huang, Y. -F., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Simultaneous+determination+of+twenty-nine+active+compounds+in+Fuzhengjiedu+granules+by+HPLC-QQQ-MS%2FMS%5BTitle%5D)+AND+%22Heliyon%22%5BJournal%5D)">Simultaneous determination of twenty-nine active compounds in Fuzhengjiedu granules by HPLC-QQQ-MS/MS.</a> Heliyon. 9 (2), e13675(2023).</li>
<li>Zhang, K. -X., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Improved+quality+control+method+for+prescriptions+of+Polygonum+capitatum+through+simultaneous+determination+of+nine+major+constituents+by+HPLC+coupled+with+triple+quadruple+mass+spectrometry%5BTitle%5D)+AND+%22Molecules%22%5BJournal%5D)">Improved quality control method for prescriptions of Polygonum capitatum through simultaneous determination of nine major constituents by HPLC coupled with triple quadruple mass spectrometry.</a> Molecules. 18 (10), 11824-11835 (2013).</li>
<li>Ding, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Comprehensive+chemical+profiling+and+quantification+of+Shexiang+xintongning+tablets+by+integrating+liquid+chromatography-mass+spectrometry+and+gas+chromatography-mass+spectrometry%5BTitle%5D)+AND+%22Arab+J+Chem%22%5BJournal%5D)">Comprehensive chemical profiling and quantification of Shexiang xintongning tablets by integrating liquid chromatography-mass spectrometry and gas chromatography-mass spectrometry.</a> Arab J Chem. 16 (3), 104527(2023).</li>
<li>Zhu, L., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((The+QuEChERS+method+coupled+to+HPLC-QQQ-MS%2FMS+for+the+determination+of+25+banned+pesticide+residues+in+ethnic+medicines%5BTitle%5D)+AND+%22Res+Sq%22%5BJournal%5D)">The QuEChERS method coupled to HPLC-QQQ-MS/MS for the determination of 25 banned pesticide residues in ethnic medicines.</a> Res Sq. , (2022).</li>
<li>Cinelli, M. A., Cline, J., Watson, T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Applications+of+liquid+chromatography-tandem+mass+spectrometry+in+natural+products+research%3A+Tropane+alkaloids+as+a+case+study%5BTitle%5D)+AND+%22J+Vis+Exp%22%5BJournal%5D)">Applications of liquid chromatography-tandem mass spectrometry in natural products research: Tropane alkaloids as a case study.</a> J Vis Exp. (205), e66620(2024).</li>
<li>Pang, Y., Huang, Y. -K., Li, F., Yang, F. -Q., Xia, Z. -N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Rapid+screening+and+evaluation+of+antioxidants+in+alkaloid+natural+products+by+capillary+electrophoresis+with+chemiluminescence+detection%5BTitle%5D)+AND+%22Anal+Methods%22%5BJournal%5D)">Rapid screening and evaluation of antioxidants in alkaloid natural products by capillary electrophoresis with chemiluminescence detection.</a> Anal Methods. 8 (35), 6545-6553 (2016).</li>
<li>Voeten, R. L. C., Ventouri, I. K., Haselberg, R., Somsen, G. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Capillary+electrophoresis%3Atrends+and+recent+advances%5BTitle%5D)+AND+%22Anal+Chem%22%5BJournal%5D)">Capillary electrophoresis:trends and recent advances.</a> Anal Chem. 90 (3), 1464-1481 (2018).</li>
<li>Pellati, F., Benvenuti, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Chromatographic+and+electrophoretic+methods+for+the+analysis+of+phenetylamine+alkaloids+in+Citrus+aurantium%5BTitle%5D)+AND+%22J+Chromatogr+A%22%5BJournal%5D)">Chromatographic and electrophoretic methods for the analysis of phenetylamine alkaloids in Citrus aurantium.</a> J Chromatogr A. 1161 (1), 71-88 (2007).</li>
<li>Cirlini, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((A+sensitive+UHPLC-ESI-MS%2FMS+method+for+the+determination+of+tropane+alkaloids+in+herbal+teas+and+extracts%5BTitle%5D)+AND+%22Food+Control%22%5BJournal%5D)">A sensitive UHPLC-ESI-MS/MS method for the determination of tropane alkaloids in herbal teas and extracts.</a> Food Control. 105, 285-291 (2019).</li>
<li>Wojakowska, A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Structural+analysis+and+profiling+of+phenolic+secondary+metabolites+of+Mexican+lupine+species+using+LC-MS+techniques%5BTitle%5D)+AND+%22Phytochemistry%22%5BJournal%5D)">Structural analysis and profiling of phenolic secondary metabolites of Mexican lupine species using LC-MS techniques.</a> Phytochemistry. 92, 71-86 (2013).</li>
<li>Shimizu, T., Watanabe, M., Fernie, A. R., Tohge, T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Targeted+LC-MS+analysis+for+plant+secondary+metabolites%5BTitle%5D)+AND+%22Methods+Mol+Biol%22%5BJournal%5D)">Targeted LC-MS analysis for plant secondary metabolites.</a> Methods Mol Biol. 1778, 171-181 (2018).</li>
<li>Mennickent, S., De Diego, M. Analytical Method Validation as the First Step in Drug Quality Control. <a target="_blank" href="http://scholar.google.com/scholar?hl=en&safe=off&q=author%3aMennickent%2C+S+%22Quality+Management+and+Quality+Control+-+New+Trends+and+Developments%22">Quality Management and Quality Control - New Trends and Developments</a>. , IntechOpen. London, UK. (2019).</li>
<li>Yang, S. H., Wang, J., Zhang, K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Validation+of+a+two-dimensional+liquid+chromatography+method+for+quality+control+testing+of+pharmaceutical+materials%5BTitle%5D)+AND+%22J+Chromatogr+A%22%5BJournal%5D)">Validation of a two-dimensional liquid chromatography method for quality control testing of pharmaceutical materials.</a> J Chromatogr A. 1492, 89-97 (2017).</li>
<li>Zhang, X., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((HPLC+with+chiral+stationary+phase+for+separation+and+kinetics+study+of+aspartic+acid+epimerization+in+peroxiredoxin+2+active+site+peptide%5BTitle%5D)+AND+%22J+Pharm+Biomed+Anal%22%5BJournal%5D)">HPLC with chiral stationary phase for separation and kinetics study of aspartic acid epimerization in peroxiredoxin 2 active site peptide.</a> J Pharm Biomed Anal. 247, 116247(2024).</li>
<li>Lu, W., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Metabolite+measurement%3A+pittfalls+to+avoid+and+practices+to+follow%5BTitle%5D)+AND+%22Annu+Rev+Biochem%22%5BJournal%5D)">Metabolite measurement: pittfalls to avoid and practices to follow.</a> Annu Rev Biochem. 86, 277-304 (2017).</li>
<li>Leopold, J., Popkova, Y., Engel, K. M., Schiller, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Recent+developments+of+useful+MALDI+matrices+for+the+mass+spectrometric+characterization+of+lipids%5BTitle%5D)+AND+%22Biomolecules%22%5BJournal%5D)">Recent developments of useful MALDI matrices for the mass spectrometric characterization of lipids.</a> Biomolecules. 8 (4), 173(2018).</li>
<li>Parasuraman, S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((An+overview+of+liquid+chromatography-mass+spectroscopy+instrumentation%5BTitle%5D)+AND+%22Pharm+Methods%22%5BJournal%5D)">An overview of liquid chromatography-mass spectroscopy instrumentation.</a> Pharm Methods. 5 (2), 47-55 (2014).</li>
<li>Alanazi, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Recent+advances+in+liquid+chromatography%E2%80%93mass+spectrometry+%28LC%E2%80%93MS%29+applications+in+biological+and+applied+sciences%5BTitle%5D)+AND+%22Anal+Sci+Adv%22%5BJournal%5D)">Recent advances in liquid chromatography–mass spectrometry (LC–MS) applications in biological and applied sciences.</a> Anal Sci Adv. 6 (1), e70024(2025).</li>
<li>Cortese, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Compensate+for+or+minimize+matrix+effects%3F+Strategies+for+overcoming+matrix+effects+in+liquid+chromatography-mass+spectrometry+technique%3A+A+tutorial+review%5BTitle%5D)+AND+%22Molecules%22%5BJournal%5D)">Compensate for or minimize matrix effects? Strategies for overcoming matrix effects in liquid chromatography-mass spectrometry technique: A tutorial review.</a> Molecules. 25 (13), 30-47 (2020).</li>
<li>Wang, L., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((The+interplay+between+herbal+medicines+and+gut+microbiota+in+metabolic+diseases%5BTitle%5D)+AND+%22Front+Pharmacol%22%5BJournal%5D)">The interplay between herbal medicines and gut microbiota in metabolic diseases.</a> Front Pharmacol. 14, 1105405(2023).</li>
<li>Musharraf, S. G., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Screening+of+inhibitors+of+angiotensin-converting+enzyme+%28ACE%29+employing+high+performance+liquid+chromatography-electrospray+ionization+triple+quadrupole+mass+spectrometry+%28HPLC-ESI-QqQ-MS%29%5BTitle%5D)+AND+%22Eur+J+Pharm+Sci%22%5BJournal%5D)">Screening of inhibitors of angiotensin-converting enzyme (ACE) employing high performance liquid chromatography-electrospray ionization triple quadrupole mass spectrometry (HPLC-ESI-QqQ-MS).</a> Eur J Pharm Sci. 101, 182-188 (2017).</li>
<li>Liu, H., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Integrating+qualitative+and+quantitative+assessments+of+Yougui+pill%2C+an+effective+traditional+Chinese+medicine%2C+by+HPLC-LTQ-orbitrap-MSn+and+UPLC-QqQ-MS%2FMS%5BTitle%5D)+AND+%22Anal+Methods%22%5BJournal%5D)">Integrating qualitative and quantitative assessments of Yougui pill, an effective traditional Chinese medicine, by HPLC-LTQ-orbitrap-MSn and UPLC-QqQ-MS/MS.</a> Anal Methods. 9 (23), 3485-3496 (2017).</li>
<li>Parks, H. M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Redirecting+tropane+alkaloid+metabolism+reveals+pyrrolidine+alkaloid+diversity+in+Atropa+belladonna%5BTitle%5D)+AND+%22New+Phytol%22%5BJournal%5D)">Redirecting tropane alkaloid metabolism reveals pyrrolidine alkaloid diversity in Atropa belladonna.</a> New Phytol. 237 (5), 1810-1825 (2023).</li>
<li>Yan, Z., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Triterpenoid+saponins+profiling+by+adducts-targeted+neutral+loss+triggered+enhanced+resolution+and+product+ion+scanning+using+triple+quadrupole+linear+ion+trap+mass+spectrometry%5BTitle%5D)+AND+%22Anal+Chim+Acta%22%5BJournal%5D)">Triterpenoid saponins profiling by adducts-targeted neutral loss triggered enhanced resolution and product ion scanning using triple quadrupole linear ion trap mass spectrometry.</a> Anal Chim Acta. 819, 56-64 (2014).</li>
<li>Sosa-Ferrera, Z., Mahugo-Santana, C., Santana-Rodríguez, J. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Analytical+methodologies+for+the+determination+of+endocrine+disrupting+compounds+in+biological+and+environmental+samples%5BTitle%5D)+AND+%22Biomed+Res+Int%22%5BJournal%5D)">Analytical methodologies for the determination of endocrine disrupting compounds in biological and environmental samples.</a> Biomed Res Int. 2013, 674838(2013).</li>
<li>Heyde, B. J., Barthel, A., Siemens, J., Mulder, I. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((A+fast+and+robust+method+for+the+extraction+and+analysis+of+quaternary+alkyl+ammonium+compounds+from+soil+and+sewage+sludge%5BTitle%5D)+AND+%22PLoS+One%22%5BJournal%5D)">A fast and robust method for the extraction and analysis of quaternary alkyl ammonium compounds from soil and sewage sludge.</a> PLoS One. 15 (8), e0237020(2020).</li>
</ol>

```html
<table><tbody><tr><td>Agilent MassHunter 소프트웨어</td><td>Agilent Technologies</td><td></td><td></td></tr><tr><td>Agilent ZORBAX SB-C18 컬럼</td><td>Agilent Technologies</td><td>880975-902</td><td>C18 역상 컬럼, 4.6 &times; 250 mm, 5 &mu;m</td></tr><tr><td>아니소다민 하이드로브로마이드</td><td>청두 글립 바이오테크놀로지 유한공사</td><td>101-31-5</td><td>HPLC>98%</td></tr><tr><td>포름산</td><td>Sigma-Aldrich</td><td>5.33002</td><td></td></tr><tr><td>동결 건조기</td><td>EYELA, 도쿄, 일본</td><td>FDU-2110</td><td></td></tr><tr><td>고속 범용 분쇄기</td><td>타이사이트 인스트루먼트 유한공사, 중국 톈진</td><td>6010210100</td><td>FW-100, 24,000 rpm, 100 g 용량, 80 메쉬</td></tr><tr><td>히오스시아민</td><td>청두 글립 바이오테크놀로지 유한공사</td><td>55449-49-5</td><td>HPLC>98%</td></tr><tr><td>메탄올</td><td>써모피셔 사이언티픽</td><td>022909.K2</td><td></td></tr><tr><td>0.22&mu;m 미세공 멤브레인</td><td>밀리포어</td><td>SLGV033RB</td><td></td></tr><tr><td>밀리-Q Direct 8 정수 시스템</td><td>밀리포어시그마, 버링턴, MA, 미국</td><td>ZRQSVP0JP</td><td></td></tr><tr><td>스코폴라민 하이드로브로마이드</td><td>청두 글립 바이오테크놀로지 유한공사</td><td>114-49-8</td><td>HPLC>98%</td></tr></tbody></table>
```

약용 식물에서 트로판 알칼로이드의 정량을 위한 효율적이고 빠른 hplc-qqq-ms 방법

An Efficient and Rapid HPLC-QQQ-MS Method for the Quantitation of Tropane Alkaloids in Medicinal Plants

HPLC-QQQ-MS 크로마토그래피 분리 및 TA 식별 표준 혼합물과 A. tanguticus 뿌리 추출물 분석을 통해 얻어진 전형적인 MRM 크로마토그램은 그림 3과 그림 4에 제시되어 있습니다. 최적화된 HPLC 조건 하에서 3가지 표적 트로판 알칼로이드인 히오시아민, 아니소다민, 스코폴라민이 20분 이내에 성공적으로 분리되어 개발된 방법의 높은 효율성을 입증했습니다. 스코폴라민과 아니소다민 간 해상도(Rs)는 8.67로, 아니소다민과 하이오시아민 간 해상도는 11.58로 확인되었다. 모든 해상도 값은 기준선 분리 기준인 1.5를 상당히 초과하여 크로마토그래피 간섭이 없음을 나타내고 정확한 정량 분석에 적합함을 확인했습니다. 모든 화합물은 날카롭고 대칭적인 피크 형태를 보였으며, 최소한의 매트릭스 간섭으로 우...

약용 식물에서 트로판 알칼로이드의 정량을 위한 효율적이고 빠른 HPLC-QQQ-MS 방법

Anisodus tanguticus is an important Tibetan medicinal plant. The tropane alkaloids (TAs), such as hyoscyamine, anisodamine, and scopolamine, exhibit ...

an-efficient-rapid-hplc-qqq-ms-method-for-quantitation-tropane

Research

연구

JoVE Journal

Chemistry

화학

실시간 고해상도 질량 분석 다변량 통계 분석을 직접 분석에 의해 식물 재료의 종을 식별하는 방법이 제공된다.

실시간 고해상도 질량 분석에 직접 분석을 사용하여 식물 소재의 신속한 높은 처리량 종 확인

여기 멀티 주입-모 세관에 따라 품질 관리와 향상 된 해상도 및 약물 남용 및 그들의 대사 산물의 대형 패널의 검출에 대 한 허용 하는 포괄적인 약물 감시 하는 높은 처리량 방법 설명 전기 이동 법 질량 분석입니다.

멀티 주입-모 세관 전기 이동 법 질량 분석을 사용 하 여 높은 처리량 및 포괄적인 약물 감시

식물에서 생체 내 뉴클레오시드/뉴클레오티드 정량화를 위한 정밀하고 재현 가능한 방법은 여기에 설명된다. 이 방법은 HPLC-MS/MS를 사용합니다.

HPLC-MS/MS를 사용하여 뉴클레오시드/뉴클레오티드 함량 측정을 위한 식물 샘플 준비

본 프로토콜은 차밭에서 PA를 생산하는 잡초의 차 샘플에서 피롤리지딘 알칼로이드(PA)의 오염을 설명합니다.

차 샘플에서 Pyrrolizidine 알칼로이드 오염의 출처 및 경로

여기에서는 동물 유래 의약품에서 트리메틸아민(TMA) 측정에 적합한 헤드스페이스 가스 크로마토그래피-탠덤 사중극자 질량분석법(HS-GC-MS/MS) 방법을 설명합니다. 이 프로토콜에는 샘플 전처리, 헤드스페이스 처리, 분석 조건, 방법론적 검증 및 동물 유래 의약품의 TMA 측정이 포함됩니다.

헤드스페이스 가스 크로마토그래피-탠덤 사중극자 질량분석법에 의한 동물 유래 의학의 트리메틸아민 검출을 위한 개선된 기술

트로판 알칼로이드의 고속 질량 분석법(MS)/질량 분석법(MS) 기반 주석 및 분류를 위한 방법을 제시하며, 이는 트로판 함유 샘플의 예비 복제 및 분리를 위한 새로운 알칼로이드의 발견 모두에 유용합니다.

천연 제품 연구에서 액체 크로마토그래피 탠덤 질량분석법의 응용 분야: 사례 연구로서의 트로판 알칼로이드

이 프로토콜은 최근 발표된 EU Commission Regulation 2023/915에 따라 식품 내 맥각 알칼로이드의 존재를 결정하기 위해 검증된 액체 크로마토그래피-이온 이동성-고분해능 질량 분석 방법을 제공합니다.

액체 크로마토그래피-트랩 이온 이동성 분광법-비행 시간 질량 분석법을 통한 식품 매트릭스에서 조절되는 맥각 알칼로이드 검출

이 프로토콜은 시약 정제 반응 조건 bioconjugate 정화 및 bioconjugate 특성화를 포함하여 말레이 미드로 단백질을 함유하는 시스테인의 바이오 콘쥬 게이션에 필요한 중요한 단계를 자세히.

단백질 Bioconjugates의 합성<em&gt;를 통해</em&gt; 시스테인 - 말레이 미드 화학

우리는 5-노르보르넨-2-카르복실산과 5-비닐-2-노르보르넨의 촉매 삽입 중합을 설명하여 매우 높은 유리 전이 온도를 가진 기능성 중합체를 형성합니다.

기능 노보 넨의 촉매 삽입 중합을위한 간단하고 효율적인 프로토콜

I 125 - 표지 지드의 합성 및 무도 클릭 반응을 이용 dibenzocyclooctyne (DBCO) - 기 - 컨쥬 게이트 13 나노 크기의 금 나노 입자의 방사성 표지에 대한 세부 절차를 설명한다.

를 사용하여 금 나노 입자의 효율적인 방사성 표지를위한 최적화 된 프로토콜<sup&gt; (125)</sup&gt; I-표시 아 지드 보철 그룹

학습하세요 약용 식물 Anisodus tanguticus에서 트로판 알칼로이드를 HPLC-QQQ-MS로 정량하는 고속 분석법을. 이 방법은 기존 HPLC의 민감도 한계를 극복하고 20분 이내에 정확한 정량이 가능합니다.

약용 식물에서 트로판 알칼로이드의 정량을 위한 효율적이고 빠른 HPLC-QQQ-MS 방법

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