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TOPICAL COLLECTIONS

Protocols for Graft Procurement: Preservation, Organ Engineering, and Educational Training
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Guest Editors

Elise Lupon

Elise Lupon

Harvard Medical School & University Côte d’Azur

<p><span style="color: black;">Dr. Elise Lupon is an Assistant Professor in Nice, France, in the Plastic and Reconstructive Surgery department (Pr Olivier Camuzard) and a Postdoctoral Research Fellow at the Massachusetts General Hospital/Harvard Medical School. She is currently concluding a PhD focusing on Tissue Engineering applications in Reconstructive surgery, and she is building a Hand allotransplantation surgery program in Nice. She joined the Vascularized Composite Allotransplantation Laboratory (Pr. Cetrulo) in 2019 and developed expertise in tissue engineering.&nbsp;In 2021, she obtained an O-1 A visa to conduct her research both in the USA and France.</span></p>

Yanis Berkane

Yanis Berkane

Massachusetts General Hospital and Harvard Medical School and University of Rennes

<p><span style="color: black;">Dr. Yanis Berkane is a Plastic and Reconstructive Surgery Resident in Rennes, France (Pr. Nicolas Bertheuil) and a Postdoctoral Research Fellow at the Massachusetts General Hospital/Harvard Medical School (CEMS, Uygun Lab). He is currently concluding a Ph.D focusing on Machine Perfusion and Tissue Engineering applications in Reconstructive surgery, which is consistent with his clinical focus areas (Microsurgical flaps, Breast reconstruction). He joined the Vascularized Composite Allotransplantation Laboratory (Pr. Cetrulo) in 2021 and developed expertise in VCA subnormothermic machine perfusion, subzero non-freezing, and tissue engineering. He aims at an academic plastic surgeon career in France, while keeping part of his research projects in the US.&nbsp;</span></p>

Alexandre G. Lellouch

Alexandre G. Lellouch

Harvard Medical School

<p><span style="color: black;">Dr. Alexandre G. Lellouch is a Board-certified plastic surgeon in Paris and co-investigator / Lecturer at MGH/ Harvard. He has extensive experience in reconstructive microsurgery. The hand and face transplantation as clinical options in the treatment of severely injured and disfigured patients led led him to dive into the field of reconstructive transplantation. Vascularized Composite allotransplantation (VCA) has emerged in recent years as the most promising treatment option for many patients afflicted with soft tissue defects that cannot be satisfactorily reconstructed using contemporary techniques. Moreover, VCA has overtaken tissue engineering by becoming a clinically viable option. Unfortunately, the prognostic in the long term is compromised due to the ineluctable development of chronic rejection and the absence of treatment available. To overcome this issue, Dr. Lellouch’s team focuses on decellularization technologies, machine perfusion, and immune tolerance induction protocols.&nbsp;</span></p>

Olivier Camuzard

Olivier Camuzard

University Côte d'Azur

<p><span style="color: black;">Pr. Olivier Camuzard is a Professor in Plastic, Reconstructive and Hand surgery. He’s the head of the Plastic, Reconstructive and Hand department in Nice, France. He has extensive experience in reconstructive microsurgery, and has specialized in hand and wrist surgery, reconstructive limb microsurgery, peripheral nerve and brachial plexus surgery, sarcoma surgery and skin tumors. He spent a year&nbsp;in Taiwan in the world most famous center for nerve reconstruction and microsurgery (</span><a href="https://www.changgung.hospital/en/about.aspx?id=11&amp;bid=1" rel="noopener noreferrer" target="_blank" style="color: black;">Chang Gung Memorial Hospital in Linkou</a><span style="color: black;">).</span><em style="color: black;"> </em></p>

Collection Overview

We propose a collection of video methods on graft harvesting for transplantation, demonstrated by experts in their respective fields. An estimated 90,000 transplants and organ donations are carried out worldwide every year. Transplantation research is one of the fastest-growing research areas. These anatomical and surgical videos will explain the key steps in harvesting solid organs and vascularized composite allografts. This collection will help demystify the complex steps involved in graft harvesting, enhance ex vivo preservation methods, such as machine perfusion and cryoprotective agent loading, before transplantation, and aid in developing tissue engineering protocols. They are also intended as a teaching aid for transplant surgeons in training.

Articles

Establishing a Swine Model to Study Uterus Dynamic Preservation and Transplantation

Establishing a Swine Model to Study Uterus Dynamic Preservation and Transplantation

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Cited by 3

2024

Abstracts

Standardized Protocol for Vascularized Composite Allograft Procurement in Penile Transplantation

Raphael Elie Cohen1,

Jonathan Cornacchini2,

Mathieu Durand3,

Nicolas Bronsard4,

Antoine Sicard5,

Olivier Camuzard2,

Flora Barthe3,

Fabien Boucher6,

Elise Lupon*7

1Department of Urology, University of Nice, Nice, France.,

2Department of Plastic and Reconstructive Surgery, Institut Universitaire Locomoteur et du Sport, Pasteur 2 Hospital, University Côte d’Azur, Nice, France,

3Department of Urology, University of Nice, Nice, France,

4Department of Orthopeadic Surgery, Institut Universitaire Locomoteur et du Sport, Pasteur 2 Hospital, University Côte d’Azur, Nice, France,

5Department of Nephrology, Dialysis and Kidney Transplantation, University Hospital of Nice, Nice & Laboratory of Molecular PhysioMedicine (LP2M), UMR 7370, CNRS, University Côte d'Azur, Nice, France,

6Plastic, Reconstructive and Aesthetic Department, Croix-Rousse Hospital, Hospices Civils de Lyon, Lyon, France,

7lupon.e@chu-nice.fr

Vascularized Composite abdominal wall Allograft: Procurement for abdominal Allotransplantation

Pierre Barbat*1,

Antoine Sicard2,

Yanis Berkane3,

Nicolas Bronsard4,

Olivier Camuzard1,

Elise Lupon1

1Department of Plastic and Reconstructive Surgery, Institut Universitaire Locomoteur et du Sport, Pasteur 2 Hospital, University Côte d’Azur, Nice, France,

2Laboratory of Molecular PhysioMedicine (LP2M), UMR 7370, CNRS, University Côte d’Azur, Nice, France,

3Department of Plastic, Reconstructive and Aesthetic Surgery, Hospital Sud, University of Rennes 1, Rennes, France,

4Department of orthopeadic Surgery, Institut Universitaire Locomoteur et du Sport, Pasteur 2 Hospital, University Côte d’Azur, Nice, France

SimLife®: A Step-by-Step Protocol Using a Pulsatile, Revascularized Cadaver Model for High-Fidelity Extremity Flap Reconstructive Surgical Training

Ioana Ivan1,

Benjamin Hertzog1,

Abdullah A Al Qurashi1,

Pharel Njessi1,

Alexis Majchrzak2,

Gregoire Micicoi1,

Olivier Camuzard1,

Luisa Nogueira3,

Nicolas Bronsard1,

Elise LUPON*1

1Institut Universitaire Locomoteur et du Sport, Pasteur 2 Hospital, Université Côte d’Azur, Nice.,

2alexis.majchrzak@etu.unice.fr,

3Faculté de Médecine, Institut Universitaire d'Anthropologie Médico-Légale, Université Côte d'Azur, Nice France

An Orthotopic High-Fidelity Porcine Model of Donation after Circulatory Death Heart Transplantation for Cardiac Allograft Assessment and Modification

Krish Dewan1,

Abby Benkert1,

Alejandro Alvarez Lobo1,

Jengwei Chen2,

Ryan Gross1,

Martha Salinas1,

Amy Evans3,

Satyanarayana Achanta4,

Sachin Mehta4,

Sharon McCartney4,

Michael Cutrone4,

Karla Rivera5,

Keely Tran6,

Smith Ngeve7,

Paul Lezberg8,

Carmelo Milano1,

Dawn Bowles*1,

Jeffrey Keenan1

11Department of Surgery, Duke University Medical Center, Durham, North Carolina ,

21Department of Surgery, Duke University Medical Center, Durham, North Carolina; 2Department of Surgery, National Taiwan University Hospital, Taipei, Taiwan ,

33Perfusion Services, Duke University Medical Center, Durham, North Carolina ,

44Department of Anesthesiology, Duke University, Durham, North Carolina,

55Campbell University, School of Osteopathic Medicine, Buies Creek, North Carolina,

66Louisiana State University School of Medicine, New Orleans, Louisiana,

77University of North Carolina School of Medicine, Chapel Hill, North Carolina,

88TransMedics, Inc., Andover, Massachusetts

Step-by-step donor lung procurement technique from brain-dead and circulatory death donors

Anitha Chandrasekhar*1

1Northwestern Medicine

Optimal preservation transportation of cardiac allografts: from optimized cold storage to normothermic ex situ perfusion

Julien Guihaire*1

1Marie Lannelongue Hospital, University of Paris Saclay, France

Adipose component transplantation – an advanced fat grafting strategy based on adipose tissue component selection for facial rejuvenation

Chongxuan Lu1,

Junrong Cai*1

1Department of Plastic Surgery, Nanfang Hospital, Southern Medical University, Guangzhou