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November 13, 2013
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The active bacterial community associated with the gut of Spodoptera littoralis, was determined by stable-isotope-probing (SIP) coupled to pyrosequencing. Using this methodology, identification of the metabolically active bacteria species within the community was done with high resolution and precision.
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Shao, Y., Arias-Cordero, E. M., Boland, W. Identification of Metabolically Active Bacteria in the Gut of the Generalist Spodoptera littoralis via DNA Stable Isotope Probing Using 13C-Glucose. J. Vis. Exp. (81), e50734, doi:10.3791/50734 (2013).
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