Methods to Study Changes in Inherent Protein Aggregation with Age in <em>Caenorhabditis elegans</em>

Nicole Groh; Ivan Gallotta; Marie C. Lechler; Chaolie Huang; Raimund Jung; Della C. David

doi:10.3791/56464

Dziennik

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Biologia

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Methods to Study Changes in Inherent Protein Aggregation with Age in Caenorhabditis elegans

JoVE Journal
Biologia

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JoVE Journal Biologia

Methods to Study Changes in Inherent Protein Aggregation with Age in Caenorhabditis elegans

Methods to Study Changes in Inherent Protein Aggregation with Age in Caenorhabditis elegans

8,431 Views

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11:57 min

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November 26, 2017

DOI:

10.3791/56464-v

DOI:

10.3791/56464-v

•

11:57 min

November 26, 2017

•

2 Views

Nicole Groh^1,2, Ivan Gallotta¹, Marie C. Lechler^1,2, Chaolie Huang¹, Raimund Jung¹, Della C. David¹

¹Protein Aggregation and Aging,German Center for Neurodegenerative Diseases (DZNE), ²Graduate School of Cellular and Molecular Neuroscience,German Center for Neurodegenerative Diseases (DZNE)

Transkrypcja

Podsumowanie

The goal of the method presented here is to explore protein aggregation during normal aging in the model organism C. elegans. The protocol represents a powerful tool to study the highly insoluble large aggregates that form with age and to determine how changes in proteostasis impact protein aggregation.