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March 07, 2018
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This protocol describes the combinatorial use of ChIP-seq, 4sU-seq, total RNA-seq, and ribosome profiling for cell lines and primary cells. It enables tracking changes in transcription-factor binding, de novo transcription, RNA processing, turnover and translation over time, and displaying the overall course of events in activated and/or rapidly changing cells.
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Davari, K., Lichti, J., Friedel, C. C., Glasmacher, E. Real-time Analysis of Transcription Factor Binding, Transcription, Translation, and Turnover to Display Global Events During Cellular Activation. J. Vis. Exp. (133), e56752, doi:10.3791/56752 (2018).
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