7,516 Views
•
10:15 min
•
December 09, 2017
DOI:
Transkrypcja
Automatically generated
Podsumowanie
Automatically generated
We describe a simplified 3D differentiation protocol for hPSCs, using defined medium and reduced growth factors, capable of generating cell aggregates with early neuroepithelial structures and positive for cerebellar-associated markers, as well as an optional 2D modification for differentiating cells as a monolayer to generate functional neurons.
Powiązane Filmy
09:07
Genetic Manipulation of Cerebellar Granule Neurons In Vitro and In Vivo to Study Neuronal Morphology and Migration
Powiązane Filmy
13652 Views
08:02
Isolation of Distinct Cell Populations from the Developing Cerebellum by Microdissection
Powiązane Filmy
9499 Views
14:19
2D and 3D Human Induced Pluripotent Stem Cell-Based Models to Dissect Primary Cilium Involvement during Neocortical Development
Powiązane Filmy
3755 Views
10:40
Scalable Generation of Mature Cerebellar Organoids from Human Pluripotent Stem Cells and Characterization by Immunostaining
Powiązane Filmy
10503 Views
09:41
Preparation and Immunostaining of Myelinating Organotypic Cerebellar Slice Cultures
Powiązane Filmy
10868 Views
N/A
Ex Vivo Myelination and Remyelination in Cerebellar Slice Cultures as a Quantitative Model for Developmental and Disease-Relevant Manipulations
Powiązane Filmy
3746 Views
06:14
Modeling Human Cerebellar Development In Vitro in 2D Structure
Powiązane Filmy
1577 Views
09:02
Validation of a Mouse Model to Disrupt LINC Complexes in a Cell-specific Manner
Powiązane Filmy
7310 Views
08:54
Cranial Neural Crest Cells Three-Dimensional In Vitro Differentiation Protocol for Multiplexed Assay
Powiązane Filmy
367 Views
01:37
Rozwarstwienie móżdżku i przygotowanie plastrów: metoda generowania wycinków tkanek z móżdżku myszy
Powiązane Filmy
1828 Views
Read Article
Cytuj ten artykuł
Holmes, D. B., Heine, V. M. Streamlined 3D Cerebellar Differentiation Protocol with Optional 2D Modification. J. Vis. Exp. (130), e56888, doi:10.3791/56888 (2017).
Copy