Here we describe a procedure for tissue clearing, fluorescent labeling, and large-scale imaging of mouse brain tissue which, thereby, enables visualization of the three-dimensional organization of cell types in the neocortex.
Yoneda, T., Sakai, S., Maruoka, H., Hosoya, T. Large-scale Three-dimensional Imaging of Cellular Organization in the Mouse Neocortex. J. Vis. Exp. (139), e58027, doi:10.3791/58027 (2018).