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January 07, 2019
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Here, we present a protocol to screen extracellular protein microarrays for identification of novel receptor-ligand interactions in high throughput. We also describe a method to enhance detection of transient protein-protein interactions by using protein-microbead complexes.
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Husain, B., Paduchuri, S., Ramani, S. R., Martinez-Martin, N. Extracellular Protein Microarray Technology for High Throughput Detection of Low Affinity Receptor-Ligand Interactions. J. Vis. Exp. (143), e58451, doi:10.3791/58451 (2019).
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