9,660 Views
•
09:16 min
•
January 07, 2019
DOI:
Transkrypcja
Automatically generated
Podsumowanie
Automatically generated
In this protocol, we describe the complete workflow for rapid isolation of extracellular vesicles from human whole blood and characterization of specific markers by fluorescence-based nanoparticle-tracking analysis. The presented results show a high level of reproducibility and can be adjusted to cell culture supernatants.
Powiązane Filmy
08:58
Characterization of Glycoproteins with the Immunoglobulin Fold by X-Ray Crystallography and Biophysical Techniques
Powiązane Filmy
12514 Views
00:05
Isolation of Tissue Extracellular Vesicles from the Liver
Powiązane Filmy
11382 Views
10:11
Imaging of Extracellular Vesicles by Atomic Force Microscopy
Powiązane Filmy
13068 Views
13:16
Characterization of Membrane Transporters by Heterologous Expression in E. coli and Production of Membrane Vesicles
Powiązane Filmy
9180 Views
10:12
Rapid and Cost-Effective RNA Extraction of Rat Pancreatic Tissue
Powiązane Filmy
9162 Views
08:46
Analysis and Specification of Starch Granule Size Distributions
Powiązane Filmy
4999 Views
06:26
Single Cell Analysis Of Transcriptionally Active Alleles By Single Molecule FISH
Powiązane Filmy
3074 Views
10:17
Rapid Determination of Antibody-Antigen Affinity by Mass Photometry
Powiązane Filmy
7329 Views
09:06
Cryo-EM and Single-Particle Analysis with Scipion
Powiązane Filmy
3654 Views
06:07
Continuous Fluorescence-Based Endonuclease-Coupled DNA Methylation Assay to Screen for DNA Methyltransferase Inhibitors
Powiązane Filmy
2440 Views
Read Article
Cytuj ten artykuł
Weber, A., Wehmeyer, J. C., Schmidt, V., Lichtenberg, A., Akhyari, P. Rapid Fluorescence-based Characterization of Single Extracellular Vesicles in Human Blood with Nanoparticle-tracking Analysis. J. Vis. Exp. (143), e58731, doi:10.3791/58731 (2019).
Copy