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September 25, 2019
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This protocol provides a method to facilitate the generation of defined heterozygous or homozygous nucleotide changes using CRISPR-CAS9 in human pluripotent stem cells.
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Cardenas-Diaz, F. L., Maguire, J. A., Gadue, P., French, D. L. Generation of Defined Genomic Modifications Using CRISPR-CAS9 in Human Pluripotent Stem Cells. J. Vis. Exp. (151), e60085, doi:10.3791/60085 (2019).
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