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December 25, 2021
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Here we present, a method to visualize ternary complex formation between three protein partners using fluorescent-tagged proteins by BiFC based FRET-FLIM assay. This method is valuable for studying protein-protein interaction complexes in vivo.
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Boora, N., Verma, V., Khurana, R., Gawande, G., Bhimrajka, S., Chaprana, K., Kapoor, M., Kapoor, S. Determination of Tripartite Interaction between Two Monomers of a MADS-box Transcription Factor and a Calcium Sensor Protein by BiFC-FRET-FLIM Assay. J. Vis. Exp. (178), e62791, doi:10.3791/62791 (2021).
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