Tracing <em>de novo</em> Lipids using Stable Isotope Labeling LC-TIMS-TOF MS/MS

Katherine D. Castro; Lilian Valadares Tose; Matthew Willetts; Melvin A. Park; Marcela Nouzova; Fernando G. Noriega; Francisco Fernandez-Lima

doi:10.3791/65590

Śledzenie lipidów de novo przy użyciu znakowania stabilnych izotopów LC-TIMS-TOF MS/MS

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Chemistry

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JoVE Journal Chemistry

Tracing de novo Lipids using Stable Isotope Labeling LC-TIMS-TOF MS/MS

Śledzenie lipidów de novo przy użyciu znakowania stabilnych izotopów LC-TIMS-TOF MS/MS

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07:12 min

August 23, 2024

DOI: 10.3791/65590-v

Katherine D. Castro¹, Lilian Valadares Tose¹, Matthew Willetts², Melvin A. Park², Marcela Nouzova³, Fernando G. Noriega^4,5, Francisco Fernandez-Lima^1,6

¹Deparment of Chemistry and Biochemistry,Florida International University, ²Bruker Daltonics Inc., ³Institute of Parasitology,Biology Centre CAS, ⁴Department of Biological Sciences,Florida International University, ⁵Department of Parasitology,University of South Bohemia, ⁶Biomolecular Sciences Institute,Florida International University

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Overview

This research presents a method for screening lipid structures through stable isotope labeling, enhancing quantification in lipidemic samples. The study addresses the gap in standardization of LC-TIMS-TOF MS/MS in lipid investigations.

Key Study Components

Area of Science

Neuroscience
Biochemistry
Mass Spectrometry

Background

Lipidemic samples vary significantly across species.
Standardization in lipid analysis is currently lacking.
LC-TIMS-TOF MS/MS is a key technique for lipid analysis.
Understanding lipid panels can aid in diverse biological studies.

Purpose of Study

To implement specific quantification methods for lipids.
To track biomass remains in lipidemic investigations.
To explore future research in epigenetics and metabolomics.

Methods Used

Stable isotope labeling for lipid quantification.
LC-TIMS-TOF MS/MS calibration procedures.
Use of DDA and DIA methods in mass spectrometry.
Analysis of diverse samples from insects to humans.

Main Results

Developed a method for improved lipid structure screening.
Identified the need for standardization in lipid analysis.
Provided a framework for future research in related fields.
Demonstrated the application of stable isotope labeling.

Conclusions

The method enhances the understanding of lipid diversity.
Standardization is crucial for advancing lipidomic studies.
Future research will expand into epigenetics and metabolomics.

Frequently Asked Questions

What is stable isotope labeling?

Stable isotope labeling is a technique used to track and quantify specific molecules, such as lipids, in biological samples.

Why is standardization important in lipid analysis?

Standardization ensures consistency and reliability in results across different studies and laboratories.

What are LC-TIMS-TOF MS/MS methods?

LC-TIMS-TOF MS/MS refers to liquid chromatography coupled with trapped ion mobility spectrometry and time-of-flight mass spectrometry, used for detailed analysis of complex mixtures.

How does this research impact future studies?

This research lays the groundwork for more precise lipid analysis and opens avenues for exploring epigenetics and metabolomics.

What types of samples were analyzed in this study?

The study analyzed a range of samples from insects to humans to demonstrate the method's applicability.

Przedstawiona tutaj jest metoda badania struktur lipidowych poprzez znakowanie stabilnych izotopów przy użyciu czasu ich retencji, mobilności i fragmentacji.

Badania te mają na celu wdrożenie bardziej szczegółowej kwantyfikacji w próbkach lipidemicznych przy użyciu znakowania stabilnymi izotopami. Pozwala nam to zrozumieć panele lipidowe w bardzo zróżnicowanych próbkach, od owadów po ludzi. Obecnie istnieje luka w tej dziedzinie dotycząca standaryzacji LC-TIMS-TOF MS/MS w badaniach lipidemicznych.

W tym celu podajemy metodę śledzenia szczątków biomasy. W przyszłości nasze badania będą obejmowały badanie epigenetyki i metabolomiki obecnych w procesie biologicznym z wykorzystaniem metod LC-TIMS-TOF MS/MS z wykorzystaniem metod DDA i DIA. Aby rozpocząć kalibrację spektrometru mas lub MS dla LC-TIMS-TOF MS/MS, otwórz oprogramowanie sterujące TIMS i na podkarcie kalibracji kliknij M over Z, wybierz mieszankę strojenia z pola rozwijanego i kliknij kalibruj w prawym dolnym rogu.

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