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Coculture Analysis of Extracellular Protein Interactions Affecting Insulin Secretion by Pancreatic Beta Cells

Article DOI: 10.3791/50365-v • 05:52 min • June 15th, 2013
June 15th, 2013 •
1, 2, 1,3
1Pediatric Diabetes Research Center, University of California, San Diego, 2Janssen Research & Development, 3Department of Medicine, University of California, San Diego

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Transcellular protein interactions are important determinants of pancreatic beta-cell function. Detailed here is a method—adapted from a coculture model of synaptogenesis—for investigating how specific transmembrane proteins influence insulin secretion. Transfected HEK293 cells express proteins of interest; beta cells do not need to be transfected or otherwise directly perturbed.

Tags

Coculture Analysis Extracellular Protein Interactions Insulin Secretion Pancreatic Beta Cells Cell-surface Proteins Transmembrane Proteins Glucose Homeostasis Knockdown Knockout Overexpression Studies MRNA And Protein Expression Beta-cell Health And Function Intracellular Domains Transcellular Interactions Insulin Secreting Capacity Responsiveness Of Beta Cells Beta-cell Lines Dissociated Primary Beta Cells Coculture Models
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