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Deriving the Time Course of Glutamate Clearance with a Deconvolution Analysis of Astrocytic Transporter Currents

Article DOI: 10.3791/50708-v • 09:42 min • August 7th, 2013
August 7th, 2013 •
1, 1
1Synaptic Physiology Section, National Institute of Neurological Disorders and Stroke, National Institutes of Health

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We describe an analytical method to estimate the lifetime of glutamate at astrocytic membranes from electrophysiological recordings of glutamate transporter currents in astrocytes.

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Glutamate Clearance Deconvolution Analysis Astrocytic Transporter Currents Glutamate Transporters Na+ Co-transport H+ Co-transport K+ Counter-transport Stoichiometric Current Whole-cell Patch-clamp Recordings Glutamate Concentration Profile Kinetics Of Glutamate Transporters Electrotonic Properties Of Astrocytic Membranes Experimental Methods Analytical Methods Waveform Of Astrocytic Transporter Currents Flash-uncaged Glutamate Synaptically-released Glutamate Central Nervous System
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