Waiting
Processando Login

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biochemistry / Characterization of Multi-subunit Protein…
JoVE Journal
Biochemistry

É necessário ter uma assinatura JoVE para assistir este

JoVE Journal Biochemistry
Characterization of Multi-subunit Protein Complexes of Human MxA Using Non-denaturing Polyacrylamide Gel-electrophoresis
Characterization of Membrane Transporters by Heterologous Expression in E. coli and Production of Membrane Vesicles
Journal (Biochemistry)
Characterization of Membrane Transporters by Heterologous Expression in E. coli and Production of Membrane Vesicles
Rapid One-step Enzymatic Synthesis and All-aqueous Purification of Trehalose Analogues
Journal (Biochemistry)
Rapid One-step Enzymatic Synthesis and All-aqueous Purification of Trehalose Analogues
 

Characterization of Multi-subunit Protein Complexes of Human MxA Using Non-denaturing Polyacrylamide Gel-electrophoresis

Article DOI: 10.3791/54683-v • 08:55 min • October 28th, 2016
October 28th, 2016 •
1,2, 1
1Institute for Medical Virology, University of Zurich, 2Friedrich Miescher Institute for Biomedical Research

Capítulos

Resumo
Automatic Translation

This article describes a simple and rapid protocol to evaluate the oligomeric state of the dynamin-like GTPase MxA protein from lysates of human cells using a combination of non-denaturing PAGE with western blot analysis.

Tags

Multi-subunit Protein Complexes Human MxA Non-denaturing Polyacrylamide Gel Electrophoresis Subunits Homooligomeric Protein Complexes Cell Lysates Cell Biology Oligomeric Status Sodium Dodecosulfate-polyacrylamide Gel Electrophoresis Cell Culture Incubator A549 Cells Virocells Growth Medium PBS Trypsin-EDTA Solution Table Top Centrifuge
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter