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Journal / Developmental Biology / Culturing of Retinal Pigment Epithelial Cells on…
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Developmental Biology

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JoVE Journal Developmental Biology
Culturing of Retinal Pigment Epithelial Cells on an Ex Vivo Model of Aged Human Bruch's Membrane
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Culturing of Retinal Pigment Epithelial Cells on an Ex Vivo Model of Aged Human Bruch's Membrane

Article DOI: 10.3791/57084-v • 08:32 min • April 12th, 2018
April 12th, 2018 •
1, 1, 1, 2, 1
1Department of Ophthalmology and Visual Science, Yale School of Medicine, 2Edward S. Harkness Eye Institute, Columbia University School of Medicine

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The goal of this protocol is to demonstrate the culturing of retinal pigment epithelial (RPE) cells on aged and/or diseased human Bruch's membrane. This method is suitable to study RPE cell behavior on a compromised extracellular matrix.

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Bruch's Membrane Retinal Pigment Epithelial Cells Ex Vivo Model Aged Human Culturing System Proteomics Study AMD Etiology RPE Sub-biology
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