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JoVE Journal Developmental Biology
Isolation, Kultur und adipogene Induktion von Neural Crest Original Adipose-Derived Stem Cells from Periaortic Adipose Tissue
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Isolation, Kultur und adipogene Induktion von Neural Crest Original Adipose-Derived Stem Cells from Periaortic Adipose Tissue

Article DOI: 10.3791/60691-v • 08:31 min • March 2nd, 2020
March 2nd, 2020 •
*1, *2, 3, 3, 4, 5, 4, 5, 1
1Department of Cardiology, Shanghai Chest Hospital, Shanghai Jiao Tong University, 2Shanghai Institute of Nutrition and Health, Shanghai Institute for Biological Sciences, Chinese Academy of Sciences, 3Shanghai Key Laboratory of Hypertension, Shanghai Institute of Hypertension, Ruijin Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, 4Department of Cardiology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, 5Department of Cardiology, Shanghai Key Laboratory of Clinical Geriatric Medicine, Huadong Hospital Affiliated to Fudan University
* These authors contributed equally

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Wir präsentieren ein Protokoll zur Isolierung, Kultur und adipogene Induktion von neuronalen Kamm abgeleiteten Adipose-abgeleiteten Stammzellen (NCADSCs) aus dem periaortischen Fettgewebe von Wnt-1 Cre+/-; Rosa26RFP/+ Mäuse. Die NCADSCs können eine leicht zugängliche Quelle von ADSCs zur Modellierung von Adipogenese oder Lipogenese in vitro sein.

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Entwicklungsbiologie Ausgabe 157 neurale Kammzelle Wnt-1 Maus periaortisches Fettgewebe adipose-abgeleitete Stromalzellen stromale Gefäßfraktion Zellkultur adipogene Induktion
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