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Cultivo de linfocitos en microgravedad simulada utilizando un sistema de cultivo celular rotativo
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Cultivo de linfocitos en microgravedad simulada utilizando un sistema de cultivo celular rotativo

Article DOI: 10.3791/63296-v • 09:28 min • August 25th, 2022
August 25th, 2022 •
1,2, 1,2,4, 1,3
1Laboratory Medicine and Pathobiology, Temerty Faculty of Medicine, University of Toronto, 2Krembil Research Institute, Toronto Western Hospital, University Health Network, 3Pathology and Lab Medicine, Hematopathology, Mount Sinai Hospital, Sinai Health Systems, 4Medical Oncology and Hematology, Princess Margaret Cancer Centre

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Esta es una guía paso a paso para usar un sistema de cultivo celular rotativo disponible comercialmente para cultivar linfocitos en microgravedad simulada utilizando recipientes de cultivo desechables especializados. Este método de cultivo se puede aplicar a cualquier cultivo celular de tipo suspensión.

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Retracción Número 186 Cultivo celular en suspensión linfocitos microgravedad simulada anulación del vector de gravedad promediada en el tiempo clinostato 2D sistema de cultivo celular rotativo dispositivo de vaso de pared giratoria
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