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Capítulo 4
Sítios de Ligação de Ligantes
Proteins are dynamic macromolecules that carry out a wide variety of essential processes; however, the activities of most proteins depend on their…
Interfaces Proteína-Proteína
Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most…
Sítio de Ligação Conservados
Many proteins’ biological role depends on their interactions with their ligands, small molecules that bind to specific locations on the protein…
A Constante de Ligação de Equilíbrio e a Força de Ligação
The equilibrium binding constant (Kb) quantifies the strength of a protein-ligand interaction. Kb can be calculated as follows when the reaction is…
Cofatores e Coenzimas
Enzymes require additional components for proper function. There are two such classes of molecules: cofactors and coenzymes. Cofactors are metallic…
Regulação Alostérica
Allosteric regulation of enzymes occurs when the binding of a molecule to a different location from the active site causes a change in enzymatic…
Ligação e Sítios de Ligação
Allosteric proteins have more than one ligand binding site; the binding of a ligand to any of these sites influences the binding of ligands to the…
Allosteric Proteins-ATCase
Binding sites linkages can regulate a protein's function.  For example, enzyme activity is often regulated through a feedback mechanism…
Transições Alostéricas Cooperativas
Cooperative allosteric transitions can occur in multimeric proteins, where each subunit of the protein has its own ligand-binding site. When a ligand…
Fosforilação
The addition or removal of phosphate groups from proteins is the most common chemical modification that regulates cellular processes. These…
Proteína Quinases e Fosfatases
Proteins undergo chemical modifications that trigger changes in the charge, structure, and conformation of the proteins. Phosphorylation,…
GTPases e sua Regulação
Guanine nucleotide-binding proteins (G-proteins), also known as GTPases, are a superfamily of proteins that regulate many cellular processes, such as…
Reguladores de Proteínas Ligadas Covalentemente
Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an…
Complexos de Proteínas com Partes Intercambiáveis
Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s…
Funções Mecânicas das Proteínas
Proteins perform many mechanical functions in a cell. These proteins can be classified into two general categories- proteins that generate mechanical…
Função da Proteína Estrutural
Structural proteins are a category of proteins responsible for functions ranging from cell shape and movement to providing support to major…
Redes de Proteínas
An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other…
Ensaio da Actividade da Proteína Quinase com ATP Radiomarcado
Protein kinases are able to govern large-scale cellular changes in response to complex arrays of stimuli, and much effort has been directed at…
Análise de arquiteturas de proteínas e complexos proteína-ligante por espectrometria de massa estrutural Integrativa
Proteins are an important class of biological macromolecules that play many key roles in cellular functions including gene expression, catalyzing…
Quantificação da dinâmica da rede de interação protéica utilizando co-imunoprecipitação multiplexada
Dynamic protein-protein interactions control cellular behavior, from motility to DNA replication to signal transduction. However, monitoring dynamic…

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