100x stock = 500 ug/mL Cytochalasin B in DMSO. Store at -20˚ C.
Strontium Chloride
10x stock = 100 mM SrCl2 in H2O. Store at room temperature.
MCZB Stock Salts
Filter sterilize 500 mL master salts (good for 3-4 months; store at 4˚ C)
HCZB Stock Salts
Start with 500 mL master salts. Add 50 mg PVA. Stir for 30-60 min and sterile filter (good for 3 months; store at 4˚ C).
PVA
Sigma
P-8136
cold-soluble
HCZB with 11% w/v PVP
Start with 20 ml HCZB medium in a 50 mL conical tube. Place 22g PVP on top of liquid. Close tube and store undisturbed at 4˚ C for 72 – 96 hrs, at which time the PVP will have entered solution. Filter sterilize through an 8 mm filter and store at 4˚ C.
PVP
ICN Biochemicals
MW 360000
Please check for additional buffers compositions in the Protocol part.