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Editorial

O 2009 Lindau Reunião ganhador do Prêmio Nobel: Roger Y. Tsien, Química 2008

Published: January 13, 2010 doi: 10.3791/1575

Summary

Bioquímico americano Roger Tsien dividiu o Prêmio Nobel 2008 em Química com Martin Chalfie e Shimomura Osamu pela descoberta e desenvolvimento da Proteína Fluorescente Verde (GFP). Tsien melhoraram drasticamente a GFP do tipo selvagem, resultando em aumento de fluorescência, fotoestabilidade aumentou, e uma mudança no pico de excitação principal a 488 nm (correspondência FITC).

References

  1. Roger Y. Tsien - Autobiography [Internet]. , The Nobel Foundation. Available from: http://nobelprize.org/nobel_prizes/chemistry/laureates/2008/tsien-autobio.html (c2003).
  2. Nobel Lecture by Roger Y. Tsien- Media Player [Internet]. , The Nobel Foundation. Available from: http://nobelprize.org/mediaplayer/index.php?id=1070 (c2003).
  3. Davenport, D., Nicol, J. A. C. Luminescence of hydromedusae. Proc. R. Soc. 144, 399-411 (1955).
  4. Shimomura, O., Johnson, F. H., Saiga, Y. Extraction, purification and properties of aequorin, a bioluminescent protein from the luminous hydromedusan. Aequorea. J. Cell. Comp. Physiol. 59, 223-229 (1962).
  5. Tsien, R. Y. New calcium indicators and buffers with high selectivity against magnesium and protons: design, synthesis, and properties of prototype structures. Biochemistry. 19 (11), 2396-2404 (1980).
  6. Heim, R., Cubitt, A., Tsien, R. Y. Improved green fluorescence. Nature. 373, 663-664 (1995).

Tags

Biologia Celular Edição 35 GFP proteína fluorescente verde IFPs água-viva PKA Calmodulina
O 2009 Lindau Reunião ganhador do Prêmio Nobel: Roger Y. Tsien, Química 2008
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Tsien, R. Y. The 2009 Lindau NobelMore

Tsien, R. Y. The 2009 Lindau Nobel Laureate Meeting: Roger Y. Tsien, Chemistry 2008. J. Vis. Exp. (35), e1575, doi:10.3791/1575 (2010).

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