JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
JoVE Journal > Biology
Summary
Protocol
Materials
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Please note that all translations are automatically generated.
Click here for the English version.
Biology
闪存冻结和Cryosectioning E12.5小鼠脑
Published: May 28, 2007
doi:
10.3791/198
D. Spencer Currle
,
Edwin S. Monuki
1
Department of Developmental and Cell Biology
,
University of California, Irvine (UCI)
Summary
这个视频展示了闪光冻结和从小鼠胚胎脑组织切片技术。使用低温恒温器的有用提示,其中包括排除故障的方法,可以用来切割,以确保由此产生的组织部分无裂缝和其他扭曲。
Protocol
修复所需的时间为4%多聚甲醛的PBS组织。 蔗糖注入组织(cryoprotection) 请在PBS W / V在2059管30%的蔗糖溶液。 在PBS冲洗组织的3倍(与摇摆〜5分钟)。 将组织30%的蔗糖溶液中。组织不会下沉。 组织放置在4℃过夜,或直至它已经沉没。 标签适当大小cryomold信息和导向。 填写cryomold与华侨城(避免气泡)。 转移组织华侨城浴和大衣与华侨城组…
Materials
Material Name
Type
Company
Catalogue Number
Comment
Tissue-Tek Cryomold
Ted Pella, Inc.
27181
O.C.T.
Ted Pella, Inc
27050
Sucrose solution
30% sucrose solution in PBS w/v
paraformaldehyde
4% paraformaldehyde in PBS
DOWNLOAD MATERIALS LIST
Tags
Flash Freezing
Cryosectioning
E12.5
Mouse Brain
Play Video
PDF
DOI
DOWNLOAD MATERIALS LIST
Cite This Article
Currle, D. S., Monuki, E. S. Flash Freezing and Cryosectioning E12.5 Mouse Brain.
J. Vis. Exp.
(4), e198, doi:10.3791/198 (2007).
Copy Citation
Download Citation
Reprints and Permissions
View Video
✖
To prove you're not a robot, please enter the text in the image below
Email:
Enable Javascript for audio controls
Enter Captcha text here:
Submit