FDG Based PET/CT Scan: A Method of Using Radiotracer for Non-invasive Imaging of Tumor Metabolism

Abstract

Source: Momcilovic M. et al., Utilizing ¹⁸F-FDG PET/CT Imaging and Quantitative Histology to Measure Dynamic Changes in the Glucose Metabolism in Mouse Models of Lung Cancer. J. Vis. Exp. (2018)

This article describes multimodality imaging of cancerous tissues with PET and CT diagnostic imaging by using a radiotracer ¹⁸F-FDG. Cancerous tissue with high glycolytic activity uptakes more ¹⁸F-FDG.

Protocol

All procedures involving animals have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.

1.¹⁸F-FDG PET and CT Imaging in Mice

CAUTION: Use protective equipment when handling radioactivity. Follow all applicable regulatory procedures when handling radioactivity.

1. Place the cage with the mice to be imaged on a warm bed at 37 °C 1 h prior to the ¹⁸F-FDG injection to reduce the brown fat consumption of ¹⁸F-FDG.
   NOTE: Fasting the mice for 4 - 16 h can he....

Materials

List of materials used in this article

Name	Company	Catalog Number	Comments
G8 PET/CT	Perkin Elmer	CLS139564	Used for 18F-FDG PET and CT imaging of mice