50 ug/mL final concentration. Store stock solution at 10 mg/ml in -20C.
Gentamicin
Reagent
Lonza
17-518Z
50 ug/mL final concentration. Store 1000x stocks at 50 mg/mL in -20C.
Pen/Strep
Reagent
Gibco
15140-122
1% final. (Also available from other companies)
Dulbecco’s Modified Eagle’s Medium
Reagent
Gibco
11966-025
Notes on media:
These uniaxial stretch experiments are prone to contamination due to the complexity of bioreactor setup. To combat this, we have tried various combinations of antibiotics and antifungal agents. The 1% fungizone usually necessary, along with a combination of antibiotics. Either use a Pen/Strep combination, OR use a combination of Kanamycin and Gentamicin (but DO NOT try using a combination/cocktail of three or more antibiotics together as it may lead to resistant mutant bacteria, and may also be detrimental to the cells).