Analysis of Cardiomyocyte Development using Immunofluorescence in Embryonic Mouse Heart

Lisa D. Wilsbacher; Shaun R. Coughlin

doi:10.3791/52644

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Embriyonik Fare Heart Immunofluorescence kullanarak kardiyomiyosit Geliştirme Analizi

Analysis of Cardiomyocyte Development using Immunofluorescence in Embryonic Mouse Heart

JoVE Journal
Gelişim Biyolojisi

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JoVE Journal Gelişim Biyolojisi

Analysis of Cardiomyocyte Development using Immunofluorescence in Embryonic Mouse Heart

Embriyonik Fare Heart Immunofluorescence kullanarak kardiyomiyosit Geliştirme Analizi

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21,133 Views

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10:56 min

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March 26, 2015

DOI:

10.3791/52644-v

DOI:

10.3791/52644-v

•

10:56 min

March 26, 2015

•

19 Views

Lisa D. Wilsbacher^1,2, Shaun R. Coughlin²

¹Feinberg Cardiovascular Research Institute,Northwestern University, ²Cardiovascular Research Institute,University of California, San Francisco

Özet

Mutations that lead to congenital heart defects benefit from in vivo investigation of cardiac structure during development, but high-resolution structural studies in the mouse embryonic heart are technically challenging. Here we present a robust immunofluorescence and image analysis method to assess cardiomyocyte-specific structures in the developing mouse heart.

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Embriyonik Fare Heart Immunofluorescence kullanarak kardiyomiyosit Geliştirme Analizi

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