Automated Quantification and Analysis of Cell Counting Procedures Using ImageJ Plugins

Jacob O'Brien; Heyam Hayder; Chun Peng

doi:10.3791/54719

ImageJ Eklentileri Kullanma Hücre Sayım Usul Otomatik Niceleme ve Analizi

JoVE Journal
Biology

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JoVE Journal Biology

Automated Quantification and Analysis of Cell Counting Procedures Using ImageJ Plugins

ImageJ Eklentileri Kullanma Hücre Sayım Usul Otomatik Niceleme ve Analizi

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49,269 Views

11:01 min

November 17, 2016

DOI: 10.3791/54719-v

Jacob O'Brien¹, Heyam Hayder¹, Chun Peng¹

¹Department of Biology,York University

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Overview

This paper presents new open-source ImageJ plugins, Cell Concentration Calculator and migration assay Counter, for quantifying hemocytometer and migration/invasion micrographs. It also details image acquisition and calibration protocols, along with the input requirements for the plugins.

Key Study Components

Area of Science

Neuroscience
Cell Biology
Image Analysis

Background

Cell counting is essential for various biological assays.
Accurate quantification aids in understanding cell behavior.
Digital analysis tools can enhance counting efficiency.
Open-source tools promote accessibility in research.

Purpose of Study

To provide a method for quick and accurate cell counts.
To facilitate quantification in in vitro motility assays.
To describe the use of ImageJ plugins for cell analysis.

Methods Used

Utilization of ImageJ plugins for cell counting.
Image acquisition using a microscope with phase contrast filters.
Calibration protocols for accurate measurements.
Setting default image capture settings in microscope software.

Main Results

Demonstrated effectiveness of the plugins in cell quantification.
Provided detailed protocols for image acquisition.
Highlighted the advantages of digital analysis tools.
Showed how to meet input requirements for the plugins.

Conclusions

The new plugins significantly improve cell counting accuracy.
They offer a user-friendly approach for researchers.
Digital tools can streamline experimental workflows.

Frequently Asked Questions

What are the main advantages of using the new plugins?

The plugins provide fast and accurate cell counts with multiple analysis tools.

How do I set up the microscope for image acquisition?

Set the illumination to maximum and use the 4x objective with phase contrast filters.

Are the plugins suitable for all types of cell assays?

Yes, they can be used for various in vitro assays, including migration and invasion assays.

Can I access the plugins for free?

Yes, the plugins are open-source and available for free.

What is the importance of calibration in this protocol?

Calibration ensures accurate measurements and reliable results in cell counting.

Is training required to use the plugins effectively?

Basic familiarity with ImageJ is helpful, but the plugins are designed to be user-friendly.

Bu makale, iki yeni açık kaynaklı ImageJ eklentisi aracılığıyla hemositometre ve migrasyon/invazyon mikrograflarının miktar tayinini açıklamaktadır: Hücre Konsantrasyonu Hesaplayıcısı ve migrasyon tahlili Sayacı. Ayrıca, görüntü alma ve kalibrasyon protokollerini açıklar ve eklentilerin giriş gereksinimlerini ayrıntılı olarak tartışır.

Bu protokolün genel amacı, bir süspansiyondaki hücrelerin veya bir istila tahlil zarındaki bir göçteki hücrelerin doğru sayımlarını hızlı bir şekilde yapmak için dijital analiz araçlarını kullanmaktır. Bu protokol, araştırmacıların yaygın teknikler ve in vitro hücre motilite deneyleri için gerekli hücre sayısını ölçmelerine yardımcı olabilir. Bu yöntemin en büyük avantajı, birden fazla filtre ve analiz aracı içerirken hızlı ve doğru hücre sayımları sağlamasıdır.

Başlamak için mikroskop aydınlatmasını maksimuma ayarlayın. 4x objektife geçin ve faz kontrast filtrelerinin kullanımda olduğundan emin olun. Ardından, mikroskop yazılımı içinde görüntü yakalama ayarlarını varsayılan değerlerine ayarlayın.

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