A Straightforward Method for Glucosinolate Extraction and Analysis with High-pressure Liquid Chromatography (HPLC)

Katharina Grosser; Nicole M. van Dam

doi:10.3791/55425

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Yüksek basınçlı Sıvı Kromatografi ile Glukosinolat Ekstraksiyon ve Analiz A Basit Yöntemi (HPLC)

A Straightforward Method for Glucosinolate Extraction and Analysis with High-pressure Liquid Chromatography (HPLC)

JoVE Journal
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JoVE Journal Kimya

A Straightforward Method for Glucosinolate Extraction and Analysis with High-pressure Liquid Chromatography (HPLC)

Yüksek basınçlı Sıvı Kromatografi ile Glukosinolat Ekstraksiyon ve Analiz A Basit Yöntemi (HPLC)

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25,065 Views

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10:09 min

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March 15, 2017

DOI:

10.3791/55425-v

DOI:

10.3791/55425-v

•

10:09 min

March 15, 2017

•

12 Views

Katharina Grosser^1,2, Nicole M. van Dam^1,2

¹German Centre for Integrative Biodiversity Research (iDiv) Halle-Jena-Leipzig, ²Institute of Ecology,Friedrich Schiller University Jena

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Özet

Here, we describe in great detail an established and robust protocol for the extraction of glucosinolates from ground plant materials. After an on-column sulfatase treatment of the extracts, the desulfoglucosinolates are eluted and analyzed by high-pressure liquid chromatography.