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Het meten van plasma membraaneiwit endocytische de tarieven door Omkeerbare biotinylatie
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Het meten van plasma membraaneiwit endocytische de tarieven door Omkeerbare biotinylatie

Article DOI: 10.3791/1669-v • 11:33 min • December 23rd, 2009
December 23rd, 2009 •
1, 1, 1
1University of Massachusetts Medical School

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Gereglementeerde endocytose regelt de cel oppervlakte-expressie niveaus van de meerderheid van de membraaneiwitten. Hier maken wij gebruik van herleidbaar, membraan impermeant biotinylatie reagentia aan de endocytische koers van de dopamine transporter (DAT), een membraaneiwit polytopic te meten. De methode maakt een directe benadering van het meten van de endocytische snelheid van de meeste plasma-membraan eiwitten.

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Cellular Biology Celbiologie membraan mensenhandel endocytose biotinylatie
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