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Sample Preparation, RNA Extraction, PCR Clean Up, and Quantification for Whole Genome Sequencing of Rabies Virus (RABV) (Video) | JoVE

A Nanopore-Based Genomic Approach to Control Rabies in Low-Income Countries

Article DOI: 10.3791/200317-v • 03:08 min • August 18th, 2023
August 18th, 2023 •
1,2, 1,3,4,5, 1,6, 1, 1, 1,6, 7,8, 3,9, 1, 1,6
1School of Biodiversity, One Health & Veterinary Medicine, University of Glasgow, 2Research Institute for Tropical Medicine, 3University of Nairobi, Institute of Tropical and Infectious Diseases, 4Tanzania Industrial Research Development Organization, 5Ifakara Health Institute, 6MRC-University of Glasgow Centre for Virus Research, 7Department of Veterinary Public health, Ahmadu Bello University, 8African Centre of Excellence for Neglected Tropical Diseases and Forensic Biotechnology, Ahmadu Bello University, 9University of Nairobi, Kenya and Center for Epidemiological Modelling and Analysis, University of Nairobi

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Sequencing van het hele genoom Snelle karakterisering Rabiësvirus Nanopore-technologie Genomische gegevens Transmissie volgen Geografische verspreiding Infectieziekten Lage- en middeninkomenslanden (LMIC's) Hond-gemedieerde hondsdolheid Vampiervleermuizen Volksgezondheid Economische zorgen Sample-to-sequence-to-interpretation Workflow Nanopore Technologie Monsterverzameling Rabiës Diagnose Whole Genome Sequencing Workflow Primer Design And Optimization Multiplex Polymerase Chain Reaction (PCR) Sequencing Bibliotheekvoorbereiding Live en offline basisoproepen Genetische afstammingsaanduiding Fylogenetische analyse Workflow-implementatie Pijplijnvalidatie Negatieve controles Genomische tools (GLUE MADDOG) Regionale en wereldwijde fylogenieën
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