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Sequencing, Live and Offline Basecalling, Analysis, and Interpretation of Rabies Virus (RABV) Genome (Video) | JoVE
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Sequencing, Live and Offline Basecalling, Analysis, and Interpretation of Rabies Virus (RABV) Genome (Video) | JoVE

A Nanopore-Based Genomic Approach to Control Rabies in Low-Income Countries

Article DOI: 10.3791/200318-v • 05:00 min • August 18th, 2023
August 18th, 2023 •
1,2, 1,3,4,5, 1,6, 1, 1, 1,6, 7,8, 3,9, 1, 1,6
1School of Biodiversity, One Health & Veterinary Medicine, University of Glasgow, 2Research Institute for Tropical Medicine, 3University of Nairobi, Institute of Tropical and Infectious Diseases, 4Tanzania Industrial Research Development Organization, 5Ifakara Health Institute, 6MRC-University of Glasgow Centre for Virus Research, 7Department of Veterinary Public health, Ahmadu Bello University, 8African Centre of Excellence for Neglected Tropical Diseases and Forensic Biotechnology, Ahmadu Bello University, 9University of Nairobi, Kenya and Center for Epidemiological Modelling and Analysis, University of Nairobi

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Helgenomsekvensering snabb karakterisering rabiesvirus nanoporteknik genomiska data överföringsspårning geografisk spridning infektionssjukdomar låg- och medelinkomstländer (LMIC) hundmedierade rabies vampyrfladdermöss folkhälsa ekonomiska problem prov-till-sekvens-till-tolkning-arbetsflöde nanoporteknik provinsamling rabiesdiagnos helgenomsekvenseringsarbetsflöde primerdesign och optimering multiplexpolymeraskedjereaktion (PCR) sekvensering Biblioteksförberedelse live och offline basanrop genetisk härstamningsbeteckning fylogenetisk analys arbetsflödesimplementering pipelinevalidering negativa kontroller genomiska verktyg (GLUE MADDOG) regionala och globala fylogenier
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