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Journal / Developmental Biology / Satellite Cell Isolation from Mouse Limb Muscles… / 使用FACS分选的小鼠卫星细胞的核酸酶分析在靶标下进行切割和释放…
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Developmental Biology

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JoVE Journal Developmental Biology
使用FACS分选的小鼠卫星细胞的核酸酶分析在靶标下进行切割和释放
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使用FACS分选的小鼠卫星细胞的核酸酶分析在靶标下进行切割和释放

Satellite Cell Isolation from Mouse Limb Muscles and Flow Cytometry Analysis

Article DOI: 10.3791/200376-v • 04:25 min • July 7th, 2023
July 7th, 2023 •
*1, *1, 1, 1, 1, 1, 1, 1
1CNRS, Inserm, IGBMC UMR 7104- UMR-S 1258, Université de Strasbourg
* These authors contributed equally

Tags

CUT&RUN分析， FACS分离的小鼠卫星细胞， 干细胞场， 小细胞群， 高效实验方案， 荧光激活细胞分选， 肢体肌肉， 结构蛋白， 碎浆， 培养基， 分散酶， I型胶原酶， 细胞过滤器， FACS缓冲液， 活力染色剂， 免疫染色卫星细胞， Triton X-100， 刀豆球蛋白A磁珠， 转录因子， 组蛋白修饰， 蛋白A-微球菌核酸酶， 染色质切割， CaCl2， DNA提取， 图书馆， 生物信息学分析
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