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Journal / Neuroscience / Culturing Human Midbrain Dopaminergic Neurons for … / Upptining av humana iPSC-härledda mDA-neuroner…
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JoVE Journal Neuroscience
Upptining av humana iPSC-härledda mDA-neuroner för sådd
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Upptining av humana iPSC-härledda mDA-neuroner för sådd

Culturing Human Midbrain Dopaminergic Neurons for Phenotypic Profiling

Article DOI: 10.3791/200465-v • 02:23 min • July 7th, 2023
July 7th, 2023 •
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Fenotypisk profilering humana stamceller dopaminerga neuroner i mellanhjärnan Parkinsons sjukdom in vitro PD-cellmodeller inducerade pluripotenta stamceller (iPSC) neuronala fenotyper fluorescerande färgningspanel kärnfärgning α-synuklein tyrosinhydroxylas (TH) mikrotubuliassocierat protein 2 (MAP2) fenotypiskt profileringsprotokoll skalbart 384-brunnsplattor automatisk vätskehantering högkapacitetsmikroskopi G2019S-mutation leucinrikt upprepat kinas 2 (LRRK2) gen LRRK2-kinashämmare PFE-360 fenotypiska förändringar flerdimensionella fenotypiska profiler klustringsanalys maskininlärningsdriven övervakad klassificering
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