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Journal / Biology / Imaging of Mitochondrial Peroxide in Living Yeast … / Semiautomated Analysis of mtHyPer7 in Yeast Cells …
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Semiautomated Analysis of mtHyPer7 in Yeast Cells with Scripts (Video) | JoVE
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Semiautomated Analysis of mtHyPer7 in Yeast Cells with Scripts (Video) | JoVE

Imaging of Mitochondrial Peroxide in Living Yeast Cells Using mtHyper7

Article DOI: 10.3791/200472-v • 05:11 min • June 2nd, 2023
June 2nd, 2023 •
1, 1,2, 2, 1,2, 1,2
1Department of Pathology and Cell Biology, Columbia University Irving Medical Center, 2Confocal and Specialized Microscopy Shared Resource in the Herbert Irving Comprehensive Cancer Center, Columbia University Irving Medical Center

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MtHyPer7 ratiometrisk biosensor mitokondriell peroxid levande jästceller mitokondriell dysfunktion neurodegenerativa sjukdomar muskuloskeletala sjukdomar cancer åldrande genetiskt kodad biosensor minimalt invasiv väteperoxid (H2O2) mitokondririktad HyPer7 (mtHyPer7) cirkulärt permuterat fluorescerande protein OxyR-proteindomän CRISPR-Cas9-system markörfritt system jästgenomintegration spinnskivans konfokalmikroskopsystem kvantitativ analys oxidativ Stress rumslig och tidsmässig information
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