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Flowcytometri og dataanalyse av murine hjernehiston posttranslasjonelle modifikasjoner
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Flowcytometri og dataanalyse av murine hjernehiston posttranslasjonelle modifikasjoner

Mouse Brain Histone Modification Quantification Using Flow Cytometry

Article DOI: 10.3791/200982-v • 02:23 min • September 15th, 2023
September 15th, 2023 •
1, 2, 1
1Department of Biochemistry and Molecular Biology, Djavad Mowafaghian Centre for Brain Health, University of British Columbia, 2Graduate Program in Neuroscience, Djavad Mowafaghian Centre for Brain Health, University of British Columbia

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Kvantifisering Global Histone Post-translasjonelle modifikasjoner Intranukleær flowcytometri Isolert Mouse Brain Microglia Gene Expression Control Chromatin Structure Posttranslational Modifications Histone Tails Acetylering Lysinrester DNA-interaksjoner Transkripsjonsfaktortilgang Bromdomeneholdige transkripsjonsaktivatorer Forbedret genuttrykk Dynamisk regulering Celledifferensiering Cellulære miljøer Stimuli Neste generasjons sekvenseringsmetoder Høy Gjennomstrømning kvantitativ måling
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