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Evaluation of the Spatial Distribution of γH2AX following Ionizing Radiation
 

Evaluation of the Spatial Distribution of γH2AX following Ionizing Radiation

Article doi: 10.3791/2203
August 7th, 2010

Summary August 7th, 2010

Microscopic analysis of γH2AX foci, which form following the phosphorylation of H2AX at Ser-139 in response to DNA double-strand breaks, has become an invaluable tool in radiation biology. Here we used an antibody to mono-methylated histone H3 at lysine 4 as an epigenetic marker of actively transcribing euchromatin, to evaluate the spatial distribution of radiation-induced γH2AX formation within the nucleus.

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