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Measuring DNA Damage and Repair in Mouse Splenocytes After Chronic In Vivo Exposure to Very Low Doses of Beta- and Gamma-Radiation
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Measuring DNA Damage and Repair in Mouse Splenocytes After Chronic In Vivo Exposure to Very Low Doses of Beta- and Gamma-Radiation

Measuring DNA Damage and Repair in Mouse Splenocytes After Chronic In Vivo Exposure to Very Low Doses of Beta- and Gamma-Radiation

DOI:
10.3791/52912-v

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11:24 min

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July 03, 2015

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, , , , ,
1Radiological Protection Research and Instrumentation,Canadian Nuclear Laboratories

Chapters

  • 00:05Title
  • 02:24Irradiation and Splenocyte Harvest
  • 04:05Splenocyte Culture Preparation
  • 05:43Irradiation Challenge
  • 06:39Immunofluorescent Labeling with Anti-gammaH2AX Antibody
  • 08:12Results: Flow Cytometric gammaH2AX Detection is Sensitive and Specific
  • 10:08Conclusion

Summary

Automatic Translation

Automatic Translation

A protocol to evaluate changes in DNA damage levels and DNA repair capacity that may be induced by chronic in vivo low dose irradiation in mouse spleen lymphocytes, by measuring phosphorylated histone H2AX, a marker of DNA double-strand breaks, using flow cytometry is presented.

Tags

Keywords: DNA DamageDNA RepairMouse SplenocytesLow-dose RadiationGamma-radiationBeta-radiationPhosphorylated Histone H2AX (γH2AX)Flow CytometryGenomic InstabilityCancer Risk

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