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Immunofluorescence Microscopy of γH2AX and 53BP1 for Analyzing the Formation and Repair of DNA Double-strand Breaks
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Cancer Research
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JoVE Journal Cancer Research
Immunofluorescence Microscopy of γH2AX and 53BP1 for Analyzing the Formation and Repair of DNA Double-strand Breaks

Immunofluorescence Microscopy of γH2AX and 53BP1 for Analyzing the Formation and Repair of DNA Double-strand Breaks

DOI:
10.3791/56617-v

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10:47 min

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November 03, 2017

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, , ,
1Department of Hematology and Oncology,Medical Faculty Mannheim of the Heidelberg University

Chapters

  • 00:05Title
  • 00:52Material Preparation
  • 03:28Mononuclear Cells Isolation by Density Gradient Centrifugation
  • 05:26Cytospin Preparation, γH2AX and 53BP1 Immunofluorescence Staining, and Analysis
  • 08:46Results: Clearly Visible Foci and DNA Repair
  • 10:02Conclusion

Summary

Automatic Translation

Automatic Translation

This manuscript provides a protocol for the analysis of DNA double-strand breaks by immunofluorescence microscopy of γH2AX and 53BP1.

Tags

Keywords: Immunofluorescence MicroscopyγH2AX53BP1DNA Double-strand BreaksCancer ResearchGenetic InstabilityCell FixationPermeabilizationBlocking SolutionBlood SampleBone Marrow Sample

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