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成蚊（冈比亚按蚊的RNAi实验议定书）
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Protocol for RNAi Assays in Adult Mosquitoes (A. gambiae)
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成蚊（冈比亚按蚊的RNAi实验议定书）

DOI:
10.3791/230-v

•

11:48 min

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July 04, 2007

•

,
1Malaria Research Institute, Bloomberg School of Public Health,Johns Hopkins University

Chapters

  • 00:01Introduction
  • 00:24Preparation of Mosquitoes and Work Area
  • 01:47injecting Mosquitoes with dsRNA
  • 08:53Treatment of Mosquitoes after Injection
  • 11:11Conclusion
  • 11:37End Credits

Summary

Automatic Translation

Automatic Translation

反向遗传方法已被证明非常有用，确定哪些基因底层电阻载体病原体在蚊子。该视频协议说明Dimopoulos实验室使用的冈比亚按蚊的蚊子，其中海港的疟疾寄生虫注入dsRNA的一个方法。操纵注射的设置和注入胸廓的dsRNA的技术说明。

Tags

RNAi AssaysAdult MosquitoesA. GambiaeReverse GeneticsVector PathogensResistance GenesDimopoulos LabDsRNA InjectionAnopheles GambiaeMalaria ParasiteInjection Setup

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