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在蚊子(埃及斑蚊)感染引起的表型测定为登革热感染病例的议定书“
JoVE Journal
Biology
This content is Free Access.
JoVE Journal Biology
Protocol for Dengue Infections in Mosquitoes (A. aegypti) and Infection Phenotype Determination
DOI:

15:25 min

July 04, 2007

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Chapters

  • 00:01Introducton
  • 00:23Critical Reagents
  • 01:05Propagate the Virus in C6/36 Cell Line
  • 02:49Prepare the Mixture of Virus and Blood
  • 04:29Feeding Mosquitoes
  • 07:25Assay of Virus Titer in Mosquito Tissue
  • 11:435 Day Incubation
  • 14:10Conclusion
  • 15:13End Credits

Summary

Automatic Translation

一旦一个基因被确定为潜在的顽固性登革热病毒,它必须进行评估,它在防止病毒感染的蚊子作用。该协议说明登革热的蚊子感染的程度如何,可以检测。成长的文化,膜喂养蚊子人体血液中的病毒,并化验在蚊子中肠病毒滴度的技术演示。

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